An R2R3-Type Transcription Factor OsMYBAS1 Regulates Seed Germination under Artificial Accelerated Aging in Transgenic Rice (Oryza sativa L.)

MYB-type transcription factors play an essential regulatory role in seed germination and the response to seedling establishment stress. This study isolated a rice R2R3-MYB factor, OsMYBAS1, functionally characterized its germination. There was no significant difference rate of each transgenic line standard test. However, compared wild type (WT) measured artificial accelerated aging test, rates overexpression lines OE-OsMYBAS1-1 OE-OsMYBAS1-2 were significantly increased by 25.0% 21.7%, respectively. In contrast, knockout mutants osmybas1-1 osmybas1-2 decreased 21.7% 33.3%, Additionally, above data indicated that OsMYBAS1 possibly plays positive Moreover, antioxidant enzyme activities OsMYBAS1-overexpressing plants enhanced 38.5% 151.0% while superoxide dismutase (SOD) activity osmybas1 27.5%, malondialdehyde (MDA) content 24.7% on average. Interestingly, expression antioxidation-related genes OsALDH3, OsAPX3, OsCATC lines, which is consistent with results. Furthermore, transcriptome sequencing determined 284 differentially expressed (DEGs), mainly involved carbohydrate metabolic process, glycerolipid metabolism, glycerophospholipid metabolism. Therefore, these findings provide valuable insight into breeding new varieties high